Description
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat IFNalpha concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IFNalpha. Samples (or Standards) and biotinylated detection antibody specific for Rat IFNalpha are added to the micro ELISA plate wells. Rat IFNalpha would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFNalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Rat IFNalpha. You can calculate the concentration of Rat IFNalpha in the samples by comparing the OD of the samples to the standard curve